پایان نامه مطالعه اثرات تداخلی روی در جذب و انتقال آهن

فهرست و منابع پایان نامه مطالعه اثرات تداخلی روی در جذب و انتقال آهن

فهرست:

فصل اول- مقدمه

(1) مقدمه. 1

1-1 متابولسیم روی.. 2

1-1-1 پیشگفتار. 2

1-1-1-2-خصوصیات فیزیکی و شیمایی روی: 2

1-1-1-3تاریخچه. 3

1-1-2متابولسیم روی.. 3

1-1-3-کمبود روی در بدن. 4

1-1-3-1چطور کمبود روی را معالجه کنیم؟ 6

1-1-4-مسموم کنندگی ZinC.. 6

1-1-6-استفاده‌های پزشکی. 8

1-1-7-دیدگاه فیزیولوژیکی. 8

1-1-7-1-عملکردها و فار موکولوژی: 8

1-1-7-2-مکانیسم فعالیت.. 8

1-1-8-محرکهای دارویی: 9

1-1-9-نتیجه. 9

1-1-9-1فعل و انفعالات.. 11

1-1-9-2مکملهای مغذی (83)   Nutritinal supplement 11

1-1-9-3-نحوه مصرف روی: 12

1-1-2-متابولیسم آهن در بدن (Iron Metabolism) 12

1-2-1-توزیع آهن در بدن: 13

1-2-2-هموگلوبین. 13

1-2-3-ذخیره آهن. 13

1-2-4-جایگاه انتقالی ‌آهن. 14

1-2-5-جذب آهن (Iron absorption) 14

1-2-5-1مکانیسم جذب آهن. 15

1-2-6-فریتین سرم (serum ferritin) 15

1-2-6-ساختمان فریتین : 15

1-2-6-2برداشت و آزاد سازی آهن توسط فریتین. 16

1-2-6-3-عمل فریتین در بدن. 16

1-2-6-4-فریتین سرم و مقدار آن در افراد طبیعی. 16

مقادیر نرمال آهن سرم. 17

1-2-7-1تغییرات روزانه در آهن سرم. 17

1-2-8-اندازه گیری مقدار آهن سرم. 17

1-2-8-1- ملاحضات کلی: 17

1-2-8-2 اندازه گیری آهن سرم با رسوب پروتئینی: 18

1-2-8-3 اندازه گیری آهن سرم بدون رسوب پروتئینی: 18

1-2-9 اندازه گیری ظرفیت پذیرش آهن سرم: 18

1-2-9- روش اول: 18

1-2-9-2-روش دوم (روش رزین): 18

1-2-9-3- روش سوم: 19

فصل دوم- - مواد و روشها

2- مواد، وسایل، روشها 21

2-1 مواد. 21

2-2- وسایل و دستگاههای آزمایشگاهی مورد استفاده: 21

3-3- روشهای دستگاهی. 21

2-4 آزمایشات تیتراسیون اسپکتروفتومتری : 22

2-4-1 تعیین طول موج  ماکزیمم: 22

2-4-2- بررسی چگونگی جذب آهن توسط آپوترانسفرین: 22

2-4-2-1 اثر غلظت مختلف آهن بر روی باندینگ با ترانسفرین. 22

2-4-2-2 اثر زمان بر روی باندینگ آهن با ترانسفرین. 22

2-4-2-3 اثر یون بیکربنات بر روی باندینگ آهن با ترانسفرین. 22

2-4-2-4 اثر سیترات بر روی باندینگ آهن با ترانسفرین. 23

2-4-2-5 اثر غلظت مختلف اکسالات بر روی باندینگ آهن با ترانسفرین: 23

2-4-2-6 اثر PH بر روی باندینگ آهن با ترانسفرین. 23

2-4-3 بررسی اثر روی.. 23

2-4-3-1 اثر غلظتهای مختلف آهن وروی بر ترانسفرین. 23

2-4-3-2 تعیین اثر غلظت مشخصی از بی کربنات بر باندینگ غلظتهای مختلف آهن با ترانسفرین  24

2-4-3-3 اثر غلظت مشخص بی کربنات بر روی باندینگ روی با ترانسفرین. 24

2-4-3-4 اثر غلظت مشخص بی کربنات بر باندینگ آهن با ترانسفرین در حضور روی: 24

2-4-3-5 اثر غلظتهای مختلف روی در باندینگ باترانسفرین در حضور یون بی کربنات   24

2-5- آزمایشات دیالیز تعادلی: 24

2-5-1 محلول‌های لازم: 25

2-5-2- طرز کار با دستگاه: 25

2-5-3- اثر روی بر برداشت آهن توسط ترانسفرین: 26

2-5-4- روش کنترل PH: 26

2-5-5- طرز اندازه گیری آهن: 26

2-5-5-1- روش کار: 26

2-5-6- تعیین ثابت باندینگ آهن با ترانسفرین. 27

فصل سوم-نتایج

3- نتایج : 30

3-1 تیتراسیون اسپکتروفتومتری: 30

3-1-1 تعیین طول موج ماکزیمم: 30

3-1-1-2- اثر روی بر روی متالوتایونین. 31

3-1-1-3 اثر روی بر روی جذب ماکزیمم اسیدهای آمینه: 31

3-1-2 بررسی چگونگی جذب آهن توسط آپوترانسفرین: 47

3-1-2-1 اثر غلظت‌های مختلف آهن بر روی باندینگ با ترانسفرین. 47

3-1-2-2 اثر زمان: 47

3-1-2-3 اثر یون بیکربنات: 47

3-1-2-4 اثر اسید سیتریک.. 47

3-1-2-6 اثر PH.. 48

3-1-3 بررسی اثر روی.. 48

3-1-3-1 اثر تغییرات غلظت روی.. 48

3-1-3-2 اثر رقابتی روی با آهن. 48

3-2 نتایج حاصل از آزمایشات دیالیز تعادلی: 49

3-2-1 تعیین ثابت باندینگ آهن به ترانسفرین: 49

فصل چهارم- بحث

بحث.. 69

آزمایشات Invitro: 69

Refrences. 71

.

منبع:

Cousins , R.J (1996) Zinc In: present knowledge in Nutrition (Ziegler, E.E.and Filer, L.j., edc.), 7 th ed., pp.293? 306. International Life Scinces Institute Press, Washington, DC.

King, J.C. & C.L. Keen (1999) Zinc. In: Modern Nutrition in Health and Disease, (Shils, M. E. Olson, J.A, Shike, M, & Ross, C.A, eds) , 9th ed., pp. 223? 240. Williams & Wilkins, Baltimore , MD.

(1,2 Prepared By): Robert J. Cousins, ph. D. Boston Family Professor of Nutrition Food Science & Human Nutrition Dept. University of Florida, 201 FSHN

Jose G.Dorea, ph.D. “Zinc and Copper in breast milk and in hom- prepared mike as function of infant’s age. "Department of Nutrition, Universidade de Brsdilia, 70910, 970 Brasilia, DF, Brzil.

Payton, K.B, Flanagan,. P.R, Stinson, E.A, chodir ker, D.P, Chamberlain , M.J, Valvberg. L.S. “Technique for determination of human zinc absorption from measurement of radioactivity in the body.” (1982 Dec 01). Journal Article, Gastroenterology, Vol/ Issue: 83:6 Departments of Medicine and Nuclear Medicine, university Hospital Lon don, Ontario, Canada.

Griffin, Ian-BAYLOR col of MED; kim, Sandra-BAYLOR Col of MED, Abrams, steven-steve; "zinc Metabolism and Zinc kinetics in children with stablecrohn's Disease (cd)" publication Acceptance data (2000.Dec 1); pub date (2001, March 31), Journal of Federation of American Societies for Experimental Biology.

William j. Walsh, “Zinc Dfeficiency, Metal Metabolism, and Behavior Disorders” (2004) Health Research Institute.

Cunningham-Rundles, C., et al., "Zinc deficiency, depressed thymic hormones and T-lymphocyte dysfunction in patients with hypogammaglobulinemia", Clin. Immunol. Immunopathol., 21:387(1981).

Good, R.A., et al., "Zinc and immunity", in Clinical, Biochemical, and Nutritional Aspects of Trace Elements, Prasad, A.S. Ed., Alan R. Liss, New York (1982)

 Walsh, W.J., Isaacson, H.R., Rahman, F., Hall, A., and Young, I.J., "Elevated blood copper:zinc ratios in assaultive young males", Neuroscience Annual Meeting, Abstract of Papers, Miami Beach, 19 9 4 (In Print).

V. Cunnane, S.C., Zinc: Clinical and Biochemical Significance, CRC Press, Inc., Boca Raton, FL (1988)

Prasad, A.S., "Deficiency of zinc in man and its toxicity", in Trace Elements in Human Health and Disease, Vol. 1, Academic Press, New York, 1 976

Prasad, A.S., "Clinical and biochemical spectrum of zinc deficiency in human subjects", in Current Topics in Nutrition and Disease, Vol 6, New York, 1982.

 Smith, J.C., Holbrook, J.T., and Danford, D.E., "Analysis and evaluation of zinc and copper in human plasma and serum", J. Amer. College of Nutr.,4:627-638(1985)

 Kleimola, V., et al, "The zinc, copper, and iron status in children with chronic diseases", in Trace Element Analytical Chemistry in Medicine and Biology, Walter de Gruyter, New York (1983).

Reding, P., DuChateau, J., and Bataille, C., "Oral zinc supplementation improves hepatic encephalopathy", Lancet, ii, 493 (1984).

 Pohit, J., Saha, K.C., and Pal, B., "A zinc tolerance test", Clin. Chim. Acta, 114:279(1981).

 Pecoud, A., Donzel, P., and Schelling, J.L., "Effects of foodstuffs on the absorption of zinc sulphate", Clin. Pharmacol. Ther 17, 469(1975)

Molokhia, M.M. and Portnoy, B., "Zinc and copper in dermatology", in Zinc and Copper in Medicine, Charles C. Thomas, Springfield, IL (1980 ).

Schmidt, K., et.al., "Determination of trace element concentrations in psoriatic

and non-psoriatic scales with special attention to zinc", in Trace Element Analytical Chemistry in Medicine and Biology, Vol. 1, Walter de Gruyter, New York (1980)

21-. McMillan, E.M., and Rowe, D., "Plasma zinc in psoriasis. Relation to surface area involvement", Br. J. Dermatol., ) 108.301(1983)

22- Ecker, R.J. and Schroeder, A.L., "Acrodermatitis and acquired zinc deficiency", Arch. Dermatol., 114:937(1978)

23- Withers, A.F., Baker, H., and Musa, M, "Plasma zinc in psoriasis", Lancet, ii:

278(1968)

24- Van Rij, A.M., "Zinc supplements in surgery", in Zinc and Copper in Medicine, Charles C. Thomas, Springfield, IL (1982)

25- Henzel, J.H., et al., "Zinc concentrations within healing wounds: significance of post-operative zincuria on availability and requirements during tissue repair",

Arch. Surg,349:357(1970)

26-Weismann, K., "Lines of Beau: Possible markers of zinc deficiency", Acta Dermatol. Venereol,57:88(1977)

27- Collipp, P.J., et al., "Zinc deficiency: Improvement in growth and growth hormone levels with oral zinc therapy", Ann. Nutr. Metab,26:287(1982).

28- Hambridge, K.M., and Walravens, P.A., "Zinc deficiency in infants and preadolescent children", in Trace Elements in Human Health and Disease, Vol. , Prasad, A.S. and Oberleas, D., Eds., Academic Press, New York (1976).

29- Golden, B.E. and Golden, M.H.N., "Effect of zinc supplementation on the dietary intake, rate of weight gain and energy cost of tissue deposition in children recovering from severe malnutrition", Am. J. Clin. Nutr,34:77(1982)

30-. Laditan, A.O. and Ette, S.I., "Plasma zinc and copper during the acute phase of protein-energy malnutrition (PEM) and after recovery", Trop. Geogr. Med, 20:422(1967)

31- . Sandstead, H.H., Prasad, A.S., et al., "Human zinc deficiency, endocrine manifestations, and response to treatment", Amer. J. Clin. Nutr,34:77(1982)

32- Heinkin, R.I., and Bradley, D.F., "Hypogeusia corrected by nickel and zinc", Life Sci,9:701(1970)

33 .Sprenger, K.B.G. et al., "Improvement of uremic neuropathy and hypogeusia by dialysate zinc supplementation: a double-blind study", Kidney Int., Suppl. 16:5315(1983)

34- Fabris N, Mocchegiani E. Zinc, Human diseases and aging. Aging 1995; 7:77-93.

35-Sandstead HH. Zinc nutrition in the united states. Ann J clin Nutr; 1973; 26: 1251-1260.

36- Hurley LS, Swenerton H. congenital Malformations resulting from Zinc deficiency in rats proc Soc Exp Biol Med 1966; 123:692-696

37-Fosmire Gy. Greeley S, Sandstood HH. Maternal and fetal response to Various sub optimal levels of Zinc intake during gestation in the rat J nutr 1977, 107:1543-1550

38-Swenerton H, Hurley LS. Zinc deficiency in rhesus and bonnet monkeys, including effects of reproduction. JNutr 1980;110:575-583.

39-. Robertson BT, Burns MJ. Zinc metabolism and the zinc deficiency syndrome in the dog. Am J Vet Res 1963;24:997.

40-. Prasad AS, Miale A, Farid Z, Sandstead HH, Schulert AR. Zinc metabolism in patients with the syndrome of iron deficiency anemia, hepatosplenomegaly, dwarfism and hypogo­nadism. JLab Clin Med 1963;61:537-549.

41-. Fraker PJ, Jardieu P, Cook J. Zinc deficiency and immune function. Arch Dermatol 1987;123:1699-1701.

42-. Jameson S. Effects of zinc deficiency in human reproduction. Acta Med Scand (Suppl. 593) 1976; 197:3-89  Fabris N, Mocchegiani E. Zinc, human diseases and aging. Aging 1995;7:77-93.

43- Sandstead HH. Understanding zinc: recent observations and interpretations. JLab Clin Med 1994;124:322-327.

44- Brandt T. Dermatitis in children with distur­bances of the general condition and the absorption of food elements. Acta Derm Venerol 1936;17:513-546.

45- Danbolt N, Closs K. Acrodermatitis enteropathica. Acta Derm Venerol 1942;23:127-169.

46- Robertson AF, Schuerger GS, Brown RR, Karp WB. Tryptophan metabolism in acrodermatitis enteropathica. JPediatr 1973;83:1012-1016.

48- Moynahan EJ, Barnes PM. Zinc deficiency and a synthetic diet for lactose intolerance. Lancet 1973;i:676-677.

49- Moynahan EJ. Acrodermatitis enteropathica: A lethal inherited human zinc-deficiency disorder. Lancet 1974;ii:399-400.

50- Braun OH, Heilmann K, Pauli W, et al. Acroder­matitis enteropathica: Recent findings con­cerning clinical features, pathogenesis, diagnosis and therapy. Europ J Pediatr 1976;121:247-261.

51- Krieger IE. Acrodermatitis enteropathica and the relationship to pellagra. Med Hypothesis 1981;7:539-547.

52- Krieger I. Picolinic acid in the treatment of disorders requiring zinc supplementation. Nutr Rev 1980;38:148-150.

53- Evans GW. Normal and abnormal zinc absorp­tion in man and animals: The tryptophan connection. Nutr Rev 1980;38:137-141.

54- Hambidge KM, Walravens PA, Casey CE, et al. Plasma zinc concentrations of breast-fed infants. JPediatr 1979;94:607-608.

55- Eckhert CD. Isolation of a protein from human milk that enhances zinc absorption in humans. Biochem Biopys Res Comm 1985; 130:264-269

56- Evans GW, Johnson PE. Characterization and quantitation of a zinc-binding ligand in human milk. Pediatr Res 1980; 14:876-880.

57- Hurley LS, Lonnerdal B. Zinc binding in human milk: citrate versus picolinate. Nutr Rev 1982;40:65-71.

58- Lonnerdal B, Stanislowski AG, Hurley LS. Isolation of a low molecular weight zinc binding ligand from human milk. Jlnorg Biochem 1980;12:71-78.

59- Song MK, Adham NF. The role of prostaglandin E-2 in zinc absorption in the rat. Am JPhysiol 1978;234: E99-E 105.

60- Song MK, Adham NF. Relationship between zinc and prostaglandin metabolisms in plasma and small intestine of rats. Am J Clin Nutr 1985;41:1201-1209.

61- Bindra GS, Gibson RS, Thompson LU. [Phytate] [calcium]/[zinc] ratios in Asian immigrant lacto-ovo vegetarian diets and their relationship to zinc nutriture. Nutr Res 1986;6:475-483.

62- Roth HP, Kirchgessner M. Utilization of zinc from picolinic or citric acid complexes in relation to dietary protein source in rats. JNutr 1985;115:1641-1649.

63- Greger JL, Mulvaney J. Absorption and tissue distribution of zinc, iron and copper by rats fed diets containing lactalbumin, soy and supple­mental sulphur-containing amino acids. JNutr 1985;115:200-210.

64- Kauwell GP, Bailey LB, Gregory JF 3rd, et al. Zinc status is not adversely affected by folic acid supplementation and zinc intake does not impair folate utilization in human subjects. J Nutr 1995;125:66-72.

65- Davidsson L, Almgren A, Sandstrom B, Hurrell RF. Zinc absorption in adult humans: the effect of iron fortification. BrJNutr 1995;74:417-425.

66- Evans GW, Grace CI, Votava HJ. A proposed mechanism of zinc absorption in the rat. Am J Physiol 1975;228:501-505.

67- Safai-Kutti S, Selin E, Larsson S, et al. Zinc therapy in children with cystic fibrosis. Beitr Infusionsther 1991;27:104-114.

68-Duisterwinkel FJ, Wolthers BG, Koopman BJ, et al. Bioavailability of orally administered zinc, using Taurizine. Pharm Weekbl [Sci] 1986;8:85-88.

69-Turnlund JR, Keyes WR, Hudson CA, et al. A stable-isotope study of zinc, copper, and iron absorption and retention by young women fed vitamin B-6-deficient diets. Am J Clin Nutr 1991;54:1059-1064.

70- Blakeborough P, Gurr MI, Salter DB. Digestion of the zinc in human milk, cow's milk and a commercial babyfood: some implications for human infant nutrition. Br J Nutr 1986;55:209-217.

71- Wapnir RA, Wang J, Exeni RA, McVicar M. Experimental evaluation of ligands for the intestinal absorption of zinc in vivo. Am J Clin Nutr 1981;34:651.

72- Flagstad T. Zinc absorption in cattle with a dietary picolinic acid supplement. JNutr 1981;111:1996-1999.

73- Evans GW, Johnson EC. Zinc absorption in rats fed a low-protein diet and a low-protein diet supplemented with tryptophan or picolinic acid. JNutr 1980;110:1076-1080.

74- Johnson WT, Evans GW. Tissue uptake of zinc in rats following the administration of zinc dipicolinate or zinc histidinate. JNutr 1982;112:914-919.

75- Seal CJ, Heaton FW. Chemical factors affecting the intestinal absorption of zinc in vitro and in vivo. BrJNutr 1983;50:317-324.

76- Seal CJ, Heaton FW. Effect of dietary picolinic acid on the metabolism of exogenous and endogenous zinc in the rat. J Nutr 1985;115:986-993.

77- Boosalis MG, Evans GW, McClain CJ. Impaired handling of orally administered zinc in pancreatic insufficiency. Am J Clin Nutr 1983;37:268-271.

78- Barrie SA, Wright JV, Pizzorno JE, Kutter E. Comparative absorption of zinc picolinate, zinc citrate and zinc gluconate in humans. Agents and Actions 1987;21:223-228.

79- Babcock AK, Henkin RI, Aamodt RL, et al. Effects of oral zinc loading on zinc metabo­lism in humans II. In vivo kinetics. Metabo­lism 1982;4:335-347.

80- Sakai F, Yoshida S, Endo S, Tomita H. Therapeu­tic efficacy of zinc picolinate in patients with taste disorders. Nippon Jibiinkoka Gakkai Kaiho 1995;98:1135-1139.

81- Zinc bioavailability of human and cow's milk. Nutr Rev 1986;44:181-183.

82-Timothy C. Birdsall, N D; Zinc picolinate: AVsorption and supplementation" alternative medicine review. Volume 1, number 1; (alt med rev 1996; 1:26-30.)

83-Abbasi AA, Prasad AS, Rabbani P, DuMouchelle E. Experimental zinc deficiency in man. Effect on testicular function. J Lab Clin Med. )1980 Sep 96(3):544-50.20. Brun JF, Dieu-Cambrezy C, Charpiat A, Fons C, Fedou C, Micallef JP, Fussellier M, Bardet L, Orsetti A. Serum zinc in highly trained adolescent gymnasts. Biol Trace Elem Res. 1995 Jan­Mar;47(1-3):273-8:30 Couzy F, Lafargue P, Guezennec CY. Zinc metabolism in the athlete: influence of training, nutrition and other factors. IntJ Sports Med. 1490 Aug :11(4):263-6.4. Gleeson M, Bishop NC. Elite athlete immunology: importance of nutrition. Int J Sports Med. 2000 May;') Suppl 1:s 44-50-5. Hunt CD, Johnson PE, Herbel J, Mullen LK. Effects of dietary zinc depletion on seminal volume and zinc loss, serum testosterone concentrations, and sperm morphology in young men. Am Clin Nutr. 1992 ,56(1):148-57.6. Krotkiewski M, Gudmundsson M, Backstrom P, Mandroukas K. Zinc and muscle strength and endurance.Acta physiol scand 1982 Nov, 116(3): 309-11.10. Lukaski HC. Magnesium, zinc, and chromium nutriture and physical activity. Am J Clin Nutr. 2000 Aug;72(2 suppl): 585 S-93508. McDonald R, Keen CL. Iron, zinc and magnesium nutrition and athletic performance. Sports Med.  1988 Mar, 5(3): 171-84. Nishi Y. Anemia and zinc deficiency in the athlete. J Am Coll Nutr 1996 Aug: 15(4): 323-4.10 t. I.. Prasad AS, Cossack ZT. Neutrophil zinc: an indicator of zinc status in man. Trans Assoc Am Physicians1982, 95: 165-76.11 1. Prasad AS. Zinc deficiency in human subjects. Prog Clin Biol Res.1983:129:1-33.12.. Shephard RI, Shek PN. Immunological hazards from nutritional imbalance in athletes. Exerc Immunol Rev. 1998, 4:22-48.13 Suboticanec K, Stavljenic A, Bilic-Pesic L, Gorajscan M, 0Gorajscan D, Brubacher G, Buzina R. Nutritional status, grip strength, and immune function in institutionalized elderly. Int J. Vitam cutr Res. 1989, 59 (1): 20-8.)

84. Mares-Perlman JA, Klein R, Klein BE, et al. Association of zinc and antioxidant nutrients with age-related maculopathy. Arch Opthalmol. 1996; 114:991-997.

85- Mc Mahon RJ, Cousins RJ. Mammalian zinc transporters. J Nutr. 1998; 128:667-670.

86- McMahon RJ, Cousins RJ. Regulation of the zinc transporter ZnT-1 by dietary zinc. Proc Natl Acad Sci USA. 1998; 95:4841-4846.

87- Mossad SB, Macknin ML, Medendorp SV, Mason PM. Zinc gluconate lozenges for treating the common cold. Ann Intern Med. 1996; 125:81-88.

88- Newsome DA, Swartz M, Leone NC. Oral zinc in macular degeneration. Arch Opthalmol. 1988; 106:192-198.

89- Noseworthy MD, Bray TM. Zinc deficiency exacerbates loss of blood-brain barrier integrity induced by hyperoxia measured by dynamic MRI. Proc Soc Exp Biol Med. 2000; 223:175-182.

90- O'Dell BL. Role of zinc in plasma membrane function. J Nutr. 2000; 130:1432S-1436S.

91- Olsen RJ, Olsen P. Zinc as a treatment for age related macular degeneration (review). J Trace Elem Exp Med. 1998; 11:137-145.

92- Prasad AS. Zinc deficiency in women, infants and children. J Am Coll Nutr. 1996; 15:113-120.

93- Prasad AS. Zinc: the biology and therapeutics of an ion. Ann Intern Med. 1996; 125:142-144.

94- Prasad AS. Discovery of human zinc deficiency and studies in an experimental human model. Am J Clin Nutr. 1991; 53:403-412.

95-Salgueiro MI, Zubillaga M, Lysionek AK, et al. Zinc an essential micronutrient: a review. Nutr Rev. 2000; 20:737-755.

96- Sandstead HH, Frederickson CJ, Penland JG. History of zinc as related to brain function. J Nutr. 2000; 130:4965-502S.

97- Sazawal S, Black RE, Bhan MK, et al. Zinc supplementation in young children with acute diarrhea in India. N Engl J Med. 1995; 333:839-844.

98- Solomons NW. Mild human zinc deficiency produces an imbalance between cell-mediated and humoral immunity. Nutr Rev. 1998; 56:27-28.

99- Sturniolo GC, Di Leo V, Barollo M, et al. The many functions of zinc in inflammatory conditions of the gastrointestinal tract. J Trace Elem Exp Med. 2000; 13:33-39.

100- Umeta M, West CE, Haidar J, et al. Zinc supplementation and stunted infants in Ethiopia: a randomized controlled trial. Lancet. 2000; 355:2021-2026.

101-Anon. Zinc lozenges reduce cold symptoms. Nutr Rev. 1997; 55:82-88.

102- Berg JM, Shi Y. The galvanization of biology: a growing appreciation for the roles of zinc. Science. 1996; l 271:1081-1085.

103-Bhutta ZA, Black RE, Brown KH, et al. Prevention of diarrhea and pneumonia by zinc supplementation in children in developing countries: Pooled analysis of randomized controlled trials. J Pediatr. 1999; 135:689-697.

104- Chandra RK. Excessive intake of zinc impairs immune response. JAMA. 1984; 252:1443-1446.

105- Cuajungco MP, Lees GJ. Zinc metabolism in the brain; relevance to human neurodegenerative disorders. Neurobiol Dis. 1997; 4:137-169.

106-Duchateau J, Delepesse G, Vrijens R, et al. Beneficial effects of oral zinc duration on the immune response of old people. Am J Med. 1981; 70:1001-1004.

107-Fabris N, Mocchegiani E. Zinc, human diseases and aging. Aging Clin Exp Res. 1995; 7:77-93.

108- Grungreiff K, Grungreiff S, Reinhold D. Zinc deficiency and hepatic encephalopathy: results of a long-term follow-up on zinc supplementation. J Trace Elem Exp Med. 2000; 13:21-31.

109-  Hambridge M. Human zinc deficiency. J Nutr. 2000; 130:1344S-1349S.

110- Jackson JL, Peterson C, Lesho E. A meta-analysis of zinc salts, lozenges and the common cold. Arch Intern Med. 1997; 157:2373-2376.

111-King JC, Keen CL. Zinc. In: Shils ME, Olson JA, Shike M, Ross AC, eds. Modern Nutrition in Health and Disease. Baltimore, MD: Williams and Wilkins; 1999:223-239.

112- Klug A, Rhodes D. "Zinc fingers": a novel protein motif for nucleic acid recognition. Trends Biochem Sci. 1987; 5:464-469.

113- Lonnerdal B. Dietary factors influencing zinc absorption. J Nutr. 2000; 130(5S Suppl): 1378S-1383S.

114- Macknin ML. Zinc lozenges for the common cold. Cleveland Clin J Med. 1999; 66:27-31.

115- Macknin ML. Piedmonte M, Calendine C, et al. Zinc gluconate lozenges for treating the common cold in children. A randomized controlled trial. JAMA. 1998; 279:1962-1967.

116- ناهید، امینی، دانشکده علوم پزشکی مشهد، (شماره 85-آ)

117- مجتبی، طبرستانی، خونشنانی پزشکی، سازمان چاپ ونشر مشهد، چاپ دوم 1366

118- John . Bernard. Menry M.D, Todd, Sanford, Davidsohn, Clinical Diagnosis and management by laboratory methods, 17th .

119- Edition vol 1 copyright 1984 by W.B. saunders company U.S.A p. 158-161

120- A.V. Hoffbrand , j.E. pettit, Essential Haematology, Copyright 1980 by Blackwell scientific publications.

121- Alexc. Sonnenwirth, ph. D. Leonard jarett, M.D Gradwohl's clinical laboratory Methods and Diagnosis Eighth Edition vol 1 copyright 1980 by the C.V Mosby company. p.850-854.

122- John D.Baver, clinical laboratory Methods . Nineth Edition, copyright by the C.V.Mosby company 1982 . p. 516-518.

.